West Nile Virus 抗体
This anti-West Nile Virus antibody is a 小鼠 单克隆 antibody detecting West Nile Virus in ELISA 和 FACS. Suitable for West Nile Virus (WNV).
Quick Overview for West Nile Virus 抗体 (ABIN7794789)
抗原
West Nile Virus (WNV)
适用
West Nile Virus (WNV)
宿主
小鼠
克隆类型
单克隆
标记
This West Nile Virus antibody is un-conjugated
应用范围
ELISA, Flow Cytometry (FACS)
克隆位点
WNV-99
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原理
- Anti-West Nile Virus (Clone: WNV-99)-Purified No Carrier Protein
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特异性
- WNV-99 activity is directed against the wing, flexible loop of NS1. During the screening phase and post characterization clone WNV-99 was found to bind to solid-phase recombinant WNV NS1 protein and to NS1 expressed on the surface of intact WNV-infected Vero cells. Plus, also engaged the hexameric form of NS1. WNV-99 was cross-reactive against Japanese Encephalitis Virus and Tick-borne Encephalitis Virus in a direct ELISA using recombinant NS1 proteins. Background West Nile Virus (WNV) is a mosquito-borne, enveloped, positive-stranded RNA flavivirus1. Flavivirus nonstructural protein NS1 has been proposed as an antibody target to avoid antibody-dependent enhancement2. NS1 is a 46-55 kDa glycoprotein that is expressed as a dimer on the cell surface and as a soluble hexamer in the extracellular space and in circulation during infection. WNV NS1 dimer consists of a ?-roll, wing, and ?-ladder.
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纯化方法
- Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
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纯度
- >=90 % monomer by analytical SEC and SDS-Page
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亚型
- IgG1
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anti-West Nile Virus (WNV) (AA 73-86) antibody
WNV 适用: West Nile Virus (WNV), 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-West Nile Virus (WNV) (AA 1-135) antibodyWNV 适用: West Nile Virus (WNV) ELISA, WB 宿主: 小鼠 Monoclonal unconjugated
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限制
- 仅限研究用
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浓度
- 1 μg/mL
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缓冲液
- This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
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储存液
- Without preservative
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储存条件
- 4 °C,-80 °C
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储存方法
- This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at <= -70°C.Avoid Repeated Freeze Thaw Cycles.
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- West Nile Virus (WNV)
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别名
- West Nile virus
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物质类
- Virus
抗原
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